Journal: BMC Oral Health
Article Title: Piezo1 participates in the tension-driven osteogenic differentiation of periodontal ligament stem cells
doi: 10.1186/s12903-025-06427-y
Figure Lengend Snippet: Characterization of hPDLSCs and changes in the cell cytoskeleton and viability after tension force application. ( A ) Morphology of the 3th generation hPDLSCs. Original magnification ×40. ( B ) After 3 weeks of osteogenic induction, hPDLSCs formed calcified nodules with a reddish coloration. Original magnification ×100. ( C ) After 2 weeks of adipogenic induction, hPDLSCs developed spherical lipid accumulations. Original magnification ×100. ( D ) The expression rates of positive markers (Strol-1, CD44, CD146, and CD90) were 98.2%, 99.2%, 99.8% and 99.6%, respectively, while the negative markers CD45 and CD34 showed positive expression rates of 0.51% and 0.94%, respectively. ( E ) The growth curve was represented as a “S shape.” ( F ) The cell viability in the 0, 1, 3, 6, 9, and 12 h groups showed no significant differences, as shown by CCK-8 assays. ( G ) Immunofluorescence staining of F-actin in hPDLSCs in the 0, 1, 3, 6, 9, 12 h groups. (red, F-actin; blue, DAPI). Scale bar = 10 μm. Data are presented as “mean ± standard deviation”
Article Snippet: Upon reaching 80–100% confluency, hPDLSCs were cultured in a pre-prepared osteogenic or adipogenic differentiation medium (Cyagen Biosciences, CA, USA) for 1–4 weeks as per the manufacturer’s protocol.
Techniques: Expressing, CCK-8 Assay, Immunofluorescence, Staining, Standard Deviation