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adipogenic differentiation medium  (Thermo Fisher)


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    Thermo Fisher adipogenic differentiation medium
    Adipogenic Differentiation Medium, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/adipogenic differentiation medium/product/Thermo Fisher
    Average 90 stars, based on 1 article reviews
    adipogenic differentiation medium - by Bioz Stars, 2026-03
    90/100 stars

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    Characterization of hPDLSCs and changes in the cell cytoskeleton and viability after tension force application. ( A ) Morphology of the 3th generation hPDLSCs. Original magnification ×40. ( B ) After 3 weeks of osteogenic induction, hPDLSCs formed calcified nodules with a reddish coloration. Original magnification ×100. ( C ) After 2 weeks of <t>adipogenic</t> induction, hPDLSCs developed spherical lipid accumulations. Original magnification ×100. ( D ) The expression rates of positive markers (Strol-1, CD44, CD146, and CD90) were 98.2%, 99.2%, 99.8% and 99.6%, respectively, while the negative markers CD45 and CD34 showed positive expression rates of 0.51% and 0.94%, respectively. ( E ) The growth curve was represented as a “S shape.” ( F ) The cell viability in the 0, 1, 3, 6, 9, and 12 h groups showed no significant differences, as shown by CCK-8 assays. ( G ) Immunofluorescence staining of F-actin in hPDLSCs in the 0, 1, 3, 6, 9, 12 h groups. (red, F-actin; blue, DAPI). Scale bar = 10 μm. Data are presented as “mean ± standard deviation”
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    Characterization of hPDLSCs and changes in the cell cytoskeleton and viability after tension force application. ( A ) Morphology of the 3th generation hPDLSCs. Original magnification ×40. ( B ) After 3 weeks of osteogenic induction, hPDLSCs formed calcified nodules with a reddish coloration. Original magnification ×100. ( C ) After 2 weeks of <t>adipogenic</t> induction, hPDLSCs developed spherical lipid accumulations. Original magnification ×100. ( D ) The expression rates of positive markers (Strol-1, CD44, CD146, and CD90) were 98.2%, 99.2%, 99.8% and 99.6%, respectively, while the negative markers CD45 and CD34 showed positive expression rates of 0.51% and 0.94%, respectively. ( E ) The growth curve was represented as a “S shape.” ( F ) The cell viability in the 0, 1, 3, 6, 9, and 12 h groups showed no significant differences, as shown by CCK-8 assays. ( G ) Immunofluorescence staining of F-actin in hPDLSCs in the 0, 1, 3, 6, 9, 12 h groups. (red, F-actin; blue, DAPI). Scale bar = 10 μm. Data are presented as “mean ± standard deviation”
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    Characterization of hPDLSCs and changes in the cell cytoskeleton and viability after tension force application. ( A ) Morphology of the 3th generation hPDLSCs. Original magnification ×40. ( B ) After 3 weeks of osteogenic induction, hPDLSCs formed calcified nodules with a reddish coloration. Original magnification ×100. ( C ) After 2 weeks of <t>adipogenic</t> induction, hPDLSCs developed spherical lipid accumulations. Original magnification ×100. ( D ) The expression rates of positive markers (Strol-1, CD44, CD146, and CD90) were 98.2%, 99.2%, 99.8% and 99.6%, respectively, while the negative markers CD45 and CD34 showed positive expression rates of 0.51% and 0.94%, respectively. ( E ) The growth curve was represented as a “S shape.” ( F ) The cell viability in the 0, 1, 3, 6, 9, and 12 h groups showed no significant differences, as shown by CCK-8 assays. ( G ) Immunofluorescence staining of F-actin in hPDLSCs in the 0, 1, 3, 6, 9, 12 h groups. (red, F-actin; blue, DAPI). Scale bar = 10 μm. Data are presented as “mean ± standard deviation”
    Adipogenic Differentiation Medium, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    PromoCell adipogenic differentiation medium 2
    Characterization of hPDLSCs and changes in the cell cytoskeleton and viability after tension force application. ( A ) Morphology of the 3th generation hPDLSCs. Original magnification ×40. ( B ) After 3 weeks of osteogenic induction, hPDLSCs formed calcified nodules with a reddish coloration. Original magnification ×100. ( C ) After 2 weeks of <t>adipogenic</t> induction, hPDLSCs developed spherical lipid accumulations. Original magnification ×100. ( D ) The expression rates of positive markers (Strol-1, CD44, CD146, and CD90) were 98.2%, 99.2%, 99.8% and 99.6%, respectively, while the negative markers CD45 and CD34 showed positive expression rates of 0.51% and 0.94%, respectively. ( E ) The growth curve was represented as a “S shape.” ( F ) The cell viability in the 0, 1, 3, 6, 9, and 12 h groups showed no significant differences, as shown by CCK-8 assays. ( G ) Immunofluorescence staining of F-actin in hPDLSCs in the 0, 1, 3, 6, 9, 12 h groups. (red, F-actin; blue, DAPI). Scale bar = 10 μm. Data are presented as “mean ± standard deviation”
    Adipogenic Differentiation Medium 2, supplied by PromoCell, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Schematic conceptualization of this study. IR activated the NF-κB signaling pathway to promote activation and maturation of DCs, amplifying inflammatory signals through the release of pro-inflammatory cytokines. The mDCs triggered oxidative stress of BMSCs, showing increased intracellular ROS levels, inhibited osteogenic differentiation, and enhanced adipogenic differentiation, which accelerated the progression of radiation-induced jaw injury. VitD3-induced tolDCs exhibited IR resistance and anti-inflammatory properties, possessing the therapeutic potential to accelerate bone regeneration in irradiated jaw defects through local administration. (IR: ionizing radiation; mDC: mature dendritic cell; tolDC: tolerogenic dendritic cell; BMSC: bone marrow mesenchymal stem cell; ROS: reactive oxygen species; vitamin D3: vitD3)

    Journal: Stem Cell Research & Therapy

    Article Title: Ionizing radiation-mediated dendritic cell maturation exacerbates inflammatory response of bone marrow mesenchymal stem cells and impairs osteogenesis in radiation-induced jaw injury

    doi: 10.1186/s13287-025-04508-x

    Figure Lengend Snippet: Schematic conceptualization of this study. IR activated the NF-κB signaling pathway to promote activation and maturation of DCs, amplifying inflammatory signals through the release of pro-inflammatory cytokines. The mDCs triggered oxidative stress of BMSCs, showing increased intracellular ROS levels, inhibited osteogenic differentiation, and enhanced adipogenic differentiation, which accelerated the progression of radiation-induced jaw injury. VitD3-induced tolDCs exhibited IR resistance and anti-inflammatory properties, possessing the therapeutic potential to accelerate bone regeneration in irradiated jaw defects through local administration. (IR: ionizing radiation; mDC: mature dendritic cell; tolDC: tolerogenic dendritic cell; BMSC: bone marrow mesenchymal stem cell; ROS: reactive oxygen species; vitamin D3: vitD3)

    Article Snippet: BMSCs were co-cultured with DC-CM and OriCell SD rat bone marrow mesenchymal stem cell adipogenic differentiation basal medium (Cyagen, USA) for 7 days.

    Techniques: Activation Assay, Irradiation

    Characterization of hPDLSCs and changes in the cell cytoskeleton and viability after tension force application. ( A ) Morphology of the 3th generation hPDLSCs. Original magnification ×40. ( B ) After 3 weeks of osteogenic induction, hPDLSCs formed calcified nodules with a reddish coloration. Original magnification ×100. ( C ) After 2 weeks of adipogenic induction, hPDLSCs developed spherical lipid accumulations. Original magnification ×100. ( D ) The expression rates of positive markers (Strol-1, CD44, CD146, and CD90) were 98.2%, 99.2%, 99.8% and 99.6%, respectively, while the negative markers CD45 and CD34 showed positive expression rates of 0.51% and 0.94%, respectively. ( E ) The growth curve was represented as a “S shape.” ( F ) The cell viability in the 0, 1, 3, 6, 9, and 12 h groups showed no significant differences, as shown by CCK-8 assays. ( G ) Immunofluorescence staining of F-actin in hPDLSCs in the 0, 1, 3, 6, 9, 12 h groups. (red, F-actin; blue, DAPI). Scale bar = 10 μm. Data are presented as “mean ± standard deviation”

    Journal: BMC Oral Health

    Article Title: Piezo1 participates in the tension-driven osteogenic differentiation of periodontal ligament stem cells

    doi: 10.1186/s12903-025-06427-y

    Figure Lengend Snippet: Characterization of hPDLSCs and changes in the cell cytoskeleton and viability after tension force application. ( A ) Morphology of the 3th generation hPDLSCs. Original magnification ×40. ( B ) After 3 weeks of osteogenic induction, hPDLSCs formed calcified nodules with a reddish coloration. Original magnification ×100. ( C ) After 2 weeks of adipogenic induction, hPDLSCs developed spherical lipid accumulations. Original magnification ×100. ( D ) The expression rates of positive markers (Strol-1, CD44, CD146, and CD90) were 98.2%, 99.2%, 99.8% and 99.6%, respectively, while the negative markers CD45 and CD34 showed positive expression rates of 0.51% and 0.94%, respectively. ( E ) The growth curve was represented as a “S shape.” ( F ) The cell viability in the 0, 1, 3, 6, 9, and 12 h groups showed no significant differences, as shown by CCK-8 assays. ( G ) Immunofluorescence staining of F-actin in hPDLSCs in the 0, 1, 3, 6, 9, 12 h groups. (red, F-actin; blue, DAPI). Scale bar = 10 μm. Data are presented as “mean ± standard deviation”

    Article Snippet: Upon reaching 80–100% confluency, hPDLSCs were cultured in a pre-prepared osteogenic or adipogenic differentiation medium (Cyagen Biosciences, CA, USA) for 1–4 weeks as per the manufacturer’s protocol.

    Techniques: Expressing, CCK-8 Assay, Immunofluorescence, Staining, Standard Deviation